Anti-PRP19 Antibody [ARC1685] (A305915)

Name: Anti-PRP19 Antibody [ARC1685]
Brand: Antibodies.com
SKU: A305915
Price: 325.0 GBP
Availability: InStock

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$485

Rabbit monoclonal [ARC1685] antibody to PRP19 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-PRP19 Antibody [ARC1685]

Description

Rabbit monoclonal [ARC1685] antibody to PRP19.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 400-504 of human PRP19 (Q9UMS4).

Sequence

FSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1685

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

hPso4, NMP200, Nuclear matrix protein 200, Pre-mRNA-processing factor 19, PRP19/PSO4 homolog, PRPF19, RING-type E3 ubiquitin transferase PRP19, Senescence evasion factor, SNEV

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-PRP19 Antibody [ARC1685] (A305915)

Western blot analysis of extracts of various cell lines, using Anti-PRP19 Antibody [ARC1685] (A305915) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Western Blot - Anti-PRP19 Antibody [ARC1685] (A305915)

Western blot analysis of extracts of Rat spleen, using Anti-PRP19 Antibody [ARC1685] (A305915) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3min.

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Immunohistochemistry - Anti-PRP19 Antibody [ARC1685] (A305915)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-PRP19 Antibody [ARC1685] (A305915) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-PRP19 Antibody [ARC1685] (A305915)

Immunohistochemistry analysis of paraffin-embedded human esophageal using Anti-PRP19 Antibody [ARC1685] (A305915) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.