This antibody recognises Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability.Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.
Applications
WB, ELISA
Dilutions
ELISA: 1:2,000 - 1:4,000, WB: 1:50 - 1:250
Reactivity
S. cerevisiae
Immunogen
Synthetic peptide corresponding to amino acids 1125-1139 of yeast Rad9 protein conjugated to Keyhole Limpet Haemocyanin.
Sequence
PSGESFRLSLDSPSK
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Concentration
500 µg/ml
Molecular Weight
Approximately 60 kDa.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rabbit anti yeast Rad9 antibody detects Saccharomyces cerevisiae Rad9, a protein involved in the DNA damage checkpoint. In S. cerevisiae this checkpoint is mainly controlled by the PI(3) kinase-like kinase (PIKK) Mec1, which is activated in response to DNA damage. Activated Mec1 hyperphosphorylates Rad9, which in turn triggers the binding of Rad53, the orthologue of mammalian Chk2. Activated Rad53 specifically targets substrates required for cell cycle arrest, gene expression and efficient DNA repair. Mutations affecting Rad9 impair checkpoint induced cell-cycle arrest and increase genomic instability. Rabbit anti yeast Rad9 antibody (AHP1294) is reactive with both the phosphorylated and non-phosphorylated forms of this protein.
Synonyms
Cell cycle checkpoint control protein, Cell cycle checkpoint control protein RAD9A, DNA repair exonuclease rad9 homolog A, hRAD 9, hRAD9, Rad 9, RAD 9A, RAD9 (S pombe) homolog, RAD9 homolog, RAD9 homolog A, Rad9a, RAD9A_HUMAN
