Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Immunogen
Trinitrophenol.
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Flow Cytometry - Rat IgG2a - Isotype Control (A284012)
Figure A. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Rat IgG2a - Isotype Control (A284012) detected with Goat anti Rat IgG2a DyLight 649. Figure B. Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975) and Anti-CD90 / Thy1 Antibody [YKIX337.217] (A282052) detected with Goat anti Rat IgG2a DyLight 649. All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Publishing research using Rat IgG2a - Isotype Control (A284012)? Please let us know so that we can list the citation on this page.
