Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Immunogen
Trinitrophenol.
Host
Rat
Clonality
Monoclonal
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat IgG2a negative control, a rat monoclonal raised against trinitrophenol, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2a test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Flow Cytometry - Rat IgG2a (PE) - Isotype Control (A282640)
Figure A. A647 conjugated Rat anti Canine CD4 and Rat IgG2a (PE) - Isotype Control (A282640). Figure B. A647 conjugated Rat anti Canine CD4 and Anti-CD5 Antibody [YKIX322.3] (PE) (A282048). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
Flow Cytometry - Rat IgG2a (PE) - Isotype Control (A282640)
Figure A. A488 conjugated Rat anti Canine CD44 and Rat IgG2a (PE) - Isotype Control (A282640). Figure B. A488 conjugated Rat anti Canine CD44 and Anti-CD45R Antibody [YKIX753.22.2] (PE) (A282060). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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