Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Immunogen
Rat IgG2b conjugated to Keyhole Limpet Haemocyanin.
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Flow Cytometry - Rat IgG2b (FITC) - Isotype Control (A282450)
Figure A. Anti-CD19 Antibody [LT19] (PE) (A280948) and Rat IgG2b (FITC) - Isotype Control (A282450). Figure B. Anti-CD19 Antibody [LT19] (PE) (A280948) and Anti-CD18 Antibody [YFC118.3] (FITC) (A282033). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock .
Flow Cytometry - Rat IgG2b (FITC) - Isotype Control (A282450)
Figure A. Anti-CD8 beta Antibody [CA15.4G2] (A279973) detected with Goat anti Mouse IgG1 DyLight 649 and Rat IgG2b (FITC) - Isotype Control (A282450). Figure B. Anti-CD8 beta Antibody [CA15.4G2] (A279973) detected with Goat anti Mouse IgG1 DyLight 649 and Anti-CD4 Antibody [YKIX302.9] (FITC) (A282041). All experiments performed on red cell lysed canine blood gated on mononuclear cells.
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