Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Immunogen
Rat IgG2b conjugated to Keyhole Limpet Haemocyanin.
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat IgG2b negative control, a rat monoclonal raised against KLH, is recommended for use as a negative control to assess the level of non-specific binding of rat IgG2b test antibodies to the surface of human and mouse cells in flow cytometry.Test results have shown that this antibody is also suitable for use as a negative control with porcine and canine cells.
Flow Cytometry - Rat IgG2b (PE) - Isotype Control (A282641)
Figure A. A647 conjugated Mouse anti Human CD4 and Rat IgG2b (PE) - Isotype Control (A282641). Figure B. A647 conjugated Mouse anti Human CD4 and Anti-CD28 Antibody [YTH913.12] (PE) (A282098). All experiments performed on human Peripheral blood lymphocytes in the presence of human SeroBlock .
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