Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Histone acetyltransferase type B subunit 2, Histone-binding protein RBBP7, Nucleosome-remodeling factor subunit RBAP46, RBBP-7, Retinoblastoma-binding protein 7, Retinoblastoma-binding protein p46
Western blot analysis of extracts of various cell lines, using Anti-RBBP7 Antibody (A6967) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Immunoprecipitation analysis of 200µg extracts of MCF7 cells using 1µg Anti-RBBP7 Antibody (A6967). Western blot was performed from the immunoprecipitate using Anti-RBBP7 Antibody (A6967) at a dilition of 1:1000.
Chromatin immunoprecipitation analysis of extracts of 293T cells, using Anti-RBBP7 Antibody (A6967) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Alternative products to Anti-RBBP7 Antibody (A15455)