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Anti-RRM2 Antibody (A357)

Western blot analysis of endogenous RRM2 in crude cell extracts. Lane 1: HeLa cells (20µg). Lane 2: MCF7 cells (20µg). Lane 3: NIH3T3 cells (20µg). Lane 4: Xenopus eggs at mitotic stage (20µg). Multiple bands are due to phosphorylation at Ser20 and/or Thr33 (human sequence). Anti-RRM2 Antibody was used at 1:1,000 dilution. Immunoprecipitation of RRM2 from CHO cells. Lane 1: Crude extract of CHO cells. Lane 2: The immunoprecipitate with Anti-RRM2 Antibody at 1:1,000 dilution. Lane 3: Supernatant of immunoprecipitation. The upper band in Lane 2 is IgG heavy chain. Anti-RRM2 Antibody was used at 1:500 dilution. Immunofluorescence staining of RRM2 protein in MCF cells with Anti-RRM2 Antibody. MCF7 cells were fixed with 4% PFA and permeabilized with 0.25% TritonX 100 and reacted with Anti-RRM2 Antibody at 1:100 dilution. Anti-Rabbit IgG Antibody (Alexa Fluor 488) was used as the secondary at 1:1,000 dilution. DNA was stained with 1.0µg/mL DAPI in TBS. Immunohistochemical staining of RRM2 in mouse testis with Anti-RRM2 Antibody. Section of formalin-fixed and paraffin embedded mouse testis was reacted with Anti-RRM2 Antibody at 1:300 dilution. Nuclear DNA was stained with DAPI (center) and merged image is shown on left. RRM2 is abundantly expressed in actively proliferating cells.
$530
Product Datasheet

Rabbit polyclonal antibody to RRM2 for WB, IP, IF and IHC-P.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.

  • Specifications

Name
Anti-RRM2 Antibody
Description
Rabbit polyclonal antibody to RRM2.
Applications
WB, IP, IF, IHC-P
Dilutions
WB: 1:1,000-1:2,000, IP: 1:300-1:1,000, IF: 1:100-1:1,000, IHC-P: 1:300
Reactivity
Human, Mouse, Rat, Hamster, Xenopus
Immunogen
Synthetic peptide (consisting of 11 amino acids) in the C-terminal region of human and mouse RRM2, conjugated with KLH.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Ribonucleoside-diphosphate reductase subunit M2, Ribonucleotide reductase small chain, Ribonucleotide reductase small subunit, RR2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western blot analysis of endogenous RRM2 in crude cell extracts. Lane 1: HeLa cells (20µg). Lane 2: MCF7 cells (20µg). Lane 3: NIH3T3 cells (20µg). Lane 4: Xenopus eggs at mitotic stage (20µg). Multiple bands are due to phosphorylation at Ser20 and/or Thr33 (human sequence). Anti-RRM2 Antibody was used at 1:1,000 dilution.Enlarge Image

Anti-RRM2 Antibody (A357)

Anti-RRM2 Antibody
Immunoprecipitation of RRM2 from CHO cells. Lane 1: Crude extract of CHO cells. Lane 2: The immunoprecipitate with Anti-RRM2 Antibody at 1:1,000 dilution. Lane 3: Supernatant of immunoprecipitation. The upper band in Lane 2 is IgG heavy chain. Anti-RRM2 Antibody was used at 1:500 dilution.Enlarge Image

Immunoprecipitation - Anti-RRM2 Antibody (A357)

Anti-RRM2 Antibody
Immunofluorescence staining of RRM2 protein in MCF cells with Anti-RRM2 Antibody. MCF7 cells were fixed with 4% PFA and permeabilized with 0.25% TritonX 100 and reacted with Anti-RRM2 Antibody at 1:100 dilution. Anti-Rabbit IgG Antibody (Alexa Fluor 488) was used as the secondary at 1:1,000 dilution. DNA was stained with 1.0µg/mL DAPI in TBS.Enlarge Image

Immunofluorescence - Anti-RRM2 Antibody (A357)

Anti-RRM2 Antibody
Immunohistochemical staining of RRM2 in mouse testis with Anti-RRM2 Antibody. Section of formalin-fixed and paraffin embedded mouse testis was reacted with Anti-RRM2 Antibody at 1:300 dilution. Nuclear DNA was stained with DAPI (center) and merged image is shown on left. RRM2 is abundantly expressed in actively proliferating cells.Enlarge Image

Anti-RRM2 Antibody (A357)

Anti-RRM2 Antibody
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Proteins predicted to interact with RRM2

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.7% Relevancy Score
97.3% Relevancy Score
95.6% Relevancy Score
95.3% Relevancy Score
95.1% Relevancy Score
95% Relevancy Score
94.7% Relevancy Score
94.5% Relevancy Score