Anti-S6K1 (phospho Thr389) Antibody (A11042)

Western blot analysis of NIH/3T3, using Anti-S6K1 (phospho Thr389) Antibody (A11042) at 1:1,000 dilution. NIH/3T3 cells were treated by 10% FBS at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

Immunofluorescence analysis of C6 cells using Anti-S6K1 (phospho Thr389) Antibody (A11042) at a dilution of 1:100. C6 cells were treated by Serum-starvation overnight at 37°C.

Immunofluorescence analysis of HeLa cells using Anti-S6K1 (phospho Thr389) Antibody (A11042) at a dilution of 1:100. HeLa cells were treated by Serum-starvation overnight at 37°C.