Western blot analysis of extracts of Jurkat cells, using Anti-SATB1 Antibody (A14984) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-SATB1 Antibody (A14984) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-SATB1 Antibody (A14984) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat thymus cells using Anti-SATB1 Antibody (A14984) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation analysis of 300µg extracts of Jurkat cells using 3µg of Anti-SATB1 Antibody (A14984). This Western blot was performed on the immunoprecipitate using Anti-SATB1 Antibody (A14984) at a dilution of 1:500.