SATB1 expression in Jurkat (A) and A431 (B) nuclear cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-SATB1 Antibody (A83671) at 0.1µg/ml (A) or 0.5µg/ml (B) and detected by chemiluminescence.
SATB1 expression in U251 (A) and Daudi (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SATB1 Antibody (A83671) at 0.5µg/ml and detected by chemiluminescence.
SATB1 expression in Human Thymus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SATB1 Antibody (A83671) at 3.75µg/ml and revealed with alkaline phosphatase (AP).
SATB1 expression in Jurkat cells analyzed by immunofluorescence. Cells immobilized on Shifix™ coverslip and permeabilized with 0.15% Triton. Staining was performed with Anti-SATB1 Antibody (A83671) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
SATB1 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SATB1 Antibody (A83671) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
