Western Blot - Anti-SATB2 Antibody [ARC2363] (A308738)
Western blot analysis of extracts of Rat brain, using Anti-SATB2 Antibody [ARC2363] (A308738) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human appendix tissue using Anti-SATB2 Antibody [ARC2363] (A308738) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil (negative control sample) using Anti-SATB2 Antibody [ARC2363] (A308738) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-SATB2 Antibody [ARC2363] (A308738) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Western Blot - Anti-SATB2 Antibody [ARC2363] (A308738)
Immunoprecipitation analysis of 300µg extracts of K-562 cells using 3µg of Anti-SATB2 Antibody [ARC2363] (A308738). This Western blot was performed on the immunoprecipitate using Anti-SATB2 Antibody [ARC2363] (A308738) at a dilution of 1:1000.
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