Anti-SEPT4 Antibody (A82695)

Name: Anti-SEPT4 Antibody
Brand: Antibodies.com
SKU: A82695
Price: 115.0 GBP
Availability: InStock

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$495

Goat polyclonal antibody to SEPT4 for ELISA, WB, IHC and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-SEPT4 Antibody

Description

Goat polyclonal antibody to SEPT4.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:64,000, WB: 1-3µg/ml, IHC: 3.75µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region, amino acids 425-435, of human SEPT4.

Sequence

C-RNKLTRESGTD

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

50 kDa

Predicted MW

53.0kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Bradeion beta, Brain protein H5, C17orf47, CE5B3 beta, Cell division control-related protein 2, hCDCREL-2, Peanut-like protein 2, PNUTL2, SEP4, Septin-4, SEPTIN4

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-SEPT4 Antibody (A82695)

SEPT4 expression in Human Heart lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SEPT4 Antibody (A82695) at 1µg/ml and detected by chemiluminescence.

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Immunohistochemistry - Anti-SEPT4 Antibody (A82695)

SEPT4 expression in Human Cerebellum analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SEPT4 Antibody (A82695) at 3.75µg/ml and revealed with alkaline phosphatase (AP).

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Flow Cytometry - Anti-SEPT4 Antibody (A82695)

SEPT4 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SEPT4 Antibody (A82695) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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