SEPT4 expression in Jurkat nuclear (A) and LNCaP (B) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SEPT4 Antibody (A83090) at 0.3µg/ml and detected by chemiluminescence.
SEPT4 expression in Human Cerebellum (A) and Mouse Brain (B) lysatess analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-SEPT4 Antibody (A83090) at 0.3µg/ml and detected by chemiluminescence.
SEPT4 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-SEPT4 Antibody (A83090) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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