This antibody recognizes an extracellular epitope of CD170, a transmembrane glycoprotein expressed strongly by neutrophils, macrophages activated during infections, monocytes, and dendritic cells. This antibody cross-reacts with SIGLEC14, whose first two Ig domains are almost identical to those of CD170.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Fusion protein composed of human CD170 extracellular domain and Fc region of human IgG1.
Host
Mouse
Clonality
Monoclonal
Clone ID
1A5
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD170, CD33 antigen-like 2, CD33L2, OB-binding protein 2, OB-BP2, OBBP2, Obesity-binding protein 2, Sialic acid-binding Ig-like lectin 5, Siglec-5
Separation of human neutrophil granulocytes (red-filled) from CD170 negative lymphocytes (black-dashed) in flow cytometry surface staining of human peripheral whole blood, labeling SIGLEC5 with Anti-SIGLEC5 Antibody [1A5] (APC) (A320790), (10 µl reagent / 100 µl of peripheral whole blood).
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Proteins predicted to interact with SIGLEC5
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.