Western Blot - Anti-SIRT3 Antibody [ARC51535] (A308370)
Western blot analysis of extracts of various cell lines, using Anti-SIRT3 Antibody [ARC51535] (A308370) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Western Blot - Anti-SIRT3 Antibody [ARC51535] (A308370)
Western blot analysis of extracts of Mouse kidney, using Anti-SIRT3 Antibody [ARC51535] (A308370) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.
Western Blot - Anti-SIRT3 Antibody [ARC51535] (A308370)
Western blot analysis of extracts from wild type (WT) and SIRT3 knockout (KO) 293T cells, using Anti-SIRT3 Antibody [ARC51535] (A308370) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
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