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Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803)

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to Smad2 (phospho Ser465 + Ser467) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Smad2 (phospho Ser465 + Ser467) Antibody
Description
Rabbit polyclonal antibody to Smad2 (phospho Ser465 + Ser467).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around S465 & S467 of human Smad2 (NP_005892.1).
Sequence
SSMS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
hMAD-2, hSMAD2, JV18-1, MAD homolog 2, Mad-related protein 2, MADH2, MADR2, Mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803)

Western blot analysis of extracts of HeLa cells, using Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803) at 1:1,000 dilution. HeLa cells were treated by TGF-beta (10ng/ml) for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
Publishing research using Anti-Smad2 (phospho Ser465 + Ser467) Antibody (A308803)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with Smad2

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score