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Anti-Smad3 Antibody (A80814)

Western Blot - Anti-Smad3 Antibody (A80814) - Antibodies.com
This product is discontinued

Anti-Smad3 Antibody (A80814) has been discontinued and is no longer available.

View all Smad3 Antibodies.

  • Specifications

Name
Anti-Smad3 Antibody
Description
Rabbit polyclonal antibody to Smad3.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 150-250 of human Smad3 (NP_005893.1).
Sequence
FPPLDDYSHSIPENTNFPAGIEPQSNIPETPPPGYLSEDGETSDHQMNHSMDAGSPNLSPNPMSPAHNNLDLQPVTYCEPAFWCSISYYELNQRVGETFHA
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Smad3 Antibody (A80814) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 Antibody (A80814)

Western blot analysis of extracts of rat ovary, using Anti-Smad3 Antibody (A80814) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Proteins predicted to interact with Smad3

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score