Anti-SMAD3 Antibody [PCRP-SMAD3-1A2] (A249270)

Flow cytometric analysis of PFA fixed HeLa cells using Anti-SMAD3 Antibody [PCRP-SMAD3-1A2] followed by Goat Anti-Mouse IgG (CF® 488) (Blue). Unstained cells (red).

Immunofluorescent analysis of PFA fixed HeLa cells stained with Anti-SMAD3 Antibody [PCRP-SMAD3-1A2] followed by Goat Anti-Mouse IgG (CF® 488) (Green). Counterstain is Phalloidin-CF® 640A (Red).

Analysis of protein array containing more than 19,000 full-length human proteins using Anti-SMAD3 Antibody [PCRP-SMAD3-1A2]. Z-Score and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt^TM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt^TM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target; a MAb is considered to be specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

SDS-PAGE analysis of Anti-SMAD3 Antibody [PCRP-SMAD3-1A2] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.