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Anti-Smad3 (phospho S423 + S425) Antibody (A80467)

Western Blot - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.com Immunohistochemistry - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.com Immunofluorescence - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.com
This product is discontinued

Anti-Smad3 (phospho S423 + S425) Antibody (A80467) has been discontinued and is no longer available.

View all Smad3 Antibodies.

  • Specifications

Name
Anti-Smad3 (phospho S423 + S425) Antibody
Description
Rabbit polyclonal antibody to Smad3 (phospho S423 + S425).
Applications
WB, IHC, IF
Dilutions
WB: 1:500-1:2000, IHC: 1:50-1:200, IF: 1:50-1:200
Reactivity
Human, Mouse
Immunogen
A phospho specific peptide corresponding to residues surrounding S423/S425 of human Smad3.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
48kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.comEnlarge Image

Western Blot - Anti-Smad3 (phospho S423 + S425) Antibody (A80467)

Western blot analysis of extracts of various cell lines, using Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) at dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Immunohistochemistry - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Smad3 (phospho S423 + S425) Antibody (A80467)

Immunohistochemistry of paraffin-embedded mouse skin using Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) at dilution of 1:100 (40x lens).
Immunofluorescence - Anti-Smad3 (phospho S423 + S425) Antibody (AP0630) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-Smad3 (phospho S423 + S425) Antibody (A80467)

Immunofluorescence analysis of A431 cells using Anti-Smad3 (phospho S423 + S425) Antibody (AP0630).

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Proteins predicted to interact with Smad3

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.8% Relevancy Score