Rabbit polyclonal antibody to Smad3 (Phospho-Ser204)
Specificity
The antibody detects endogenous level of Smad3 only when phosphorylated at serine 204.
Applications
WB
Reactivity
Human, Mouse
Immunogen
Peptide sequence around phosphorylation site of serine204(A-G-S(p)-P-N) derived from Human Smad3 .
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20˚C / 1 year
Synonyms
hMAD-3, hSMAD3, JV15-2, MAD homolog 3, Mad3, MADH3, Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3
Western blot analysis of extracts from NIH-3T3 cells treated with Serum using Phospho-Smad3 (Ser204) antibody #11916.The lane on the right is treated with the antigen-specific peptide.
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