SNX1 expression in HeLa (A) and U2OS (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-SNX1 Antibody (A82618) at 1µg/ml (A) or 0.1µg/ml (B) and detected by chemiluminescence.
SNX1 expression in Human Uterus analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-SNX1 Antibody (A82618) at 3.8µg/ml and revealed with alkaline phosphatase (AP).
SNX1 expression in formaldehyde-fixed SH5Y5Y analyzed by immunocytochemistry. Staining was performed with Anti-SNX1 Antibody (A82618) at 5µg/ml and detected with Alexa 488 in confocal microscopy.
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