The antibody recognizes an epitope included within amino acids 8-23 of STAT1, a 91 kDa transcriptional factor involved in a variety of systems including antiviral responses and interferon alpha (IFN-alpha) and gamma (IFN-gamma) signal transduction.
Western blotting analysis of human STAT1 using Anti-STAT1 Antibody [SM2] (A85902) on lysates of HeLa, MCF-7, and Cos-7 cell lines under reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-STAT1 followed by IRDye800-conjugated anti-mouse secondary antibody. STAT1 was detected at approximately 90 kDa.
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