The antibody detects endogenous level of Synaptotagmin 1 / 2 only when phosphorylated at threonine202 / 199.
Applications
IF
Dilutions
Immunofluorescence: 1:100~1:200
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 202 / 199 (R-K-T(p)-L-N) derived from Human Synaptotagmin 1 / 2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Predicted MW
46kd
Formulation
Supplied at 1.0mg / mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage
Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.
Synonyms
p65, SVP65, Synaptotagmin I, Synaptotagmin-1, SYT, SYT1, SytI
Immunofluorescence staining of methanol-fixed Hela cells using Synaptotagmin 1/2 (Phospho-Thr202/199) Antibody #11210.
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