Anti-TAK1 Antibody (A305052)

Western blot analysis of rat liver lysate showing detection of ~67.2 kDa TAK1 protein using Anti-TAK1 Antibody (A305052) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~67.2 kDa. Other Band(s): ~40 kDa potential degradation product.

Immunohistochemistry analysis of human brain, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-TAK1 Antibody (A305052) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X.