Rabbit polyclonal antibody to TAK1 (Phospho-Thr184)
Specificity
The antibody detects endogenous levels of TAK1 only when phosphorylated at threonine 184.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 184 (I-Q-T(p)-H-M) derived from Human TAK1.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1 mg/ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from HepG2 cells, treated with TNF (20ng/ml, 5mins), using TAK1 (Phospho-Thr184) antibody #12153. The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from 293 cells (Lane 2), using TAK1 (Phospho-Thr184) Antibody #12153. The lane on the left is treated with synthesized peptide.
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