Western blot analysis of mouse liver lysate showing detection of ~71.3 kDa TCF4 protein using Anti-TCF4 Antibody (A304927) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: mouse liver lysate. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP:IgG at 1:3000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes at room temperature. Predicted/Observed Size: ~71.3 kDa. Other Band(s): ~45 kDa isoform.
Immunohistochemistry analysis of human kidney, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-TCF4 Antibody (A304927) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 10X. HRP-DAB Detection.
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