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Anti-TIM 3 Antibody (A14025)

Western Blot - Anti-TIM 3 Antibody (A14025) - Antibodies.com Immunohistochemistry - Anti-TIM 3 Antibody (A14025) - Antibodies.com Immunohistochemistry - Anti-TIM 3 Antibody (A14025) - Antibodies.com
$355
Product Datasheet

Rabbit polyclonal antibody to TIM 3 for WB and IHC.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-TIM 3 Antibody
Description
Rabbit polyclonal antibody to TIM 3.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 22-202 of human TIM-3/HAVCR2 (NP_116171.3).
Sequence
SEVEYRAEVGQNAYLPCFYTPAAPGNLVPVCWGKGACPVFECGNVVLRTDERDVNYWTSRYWLNGDFRKGDVSLTIENVTLADSGIYCCRIQIPGIMNDEKFNLKLVIKPAKVTPAPTRQRDFTAAFPRMLTTRGHGPAETQTLGSLPDINLTQISTLANELRDSRLANDLRDSGATIRIG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Molecular Weight
45 - 70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIM3, TIMD-3, TIMD3
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-TIM 3 Antibody (A14025) - Antibodies.comEnlarge Image

Western Blot - Anti-TIM 3 Antibody (A14025)

Western blot analysis of extracts of various cell lines, using Anti-TIM 3 Antibody (A14025) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 5s.
Immunohistochemistry - Anti-TIM 3 Antibody (A14025) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-TIM 3 Antibody (A14025)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-TIM 3 Antibody (A14025) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-TIM 3 Antibody (A14025) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-TIM 3 Antibody (A14025)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-TIM 3 Antibody (A14025) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Publishing research using Anti-TIM 3 Antibody (A14025)? Please let us know so that we can list the citation on this page.

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Proteins predicted to interact with TIM 3

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.8% Relevancy Score
99.5% Relevancy Score
99.1% Relevancy Score
98.8% Relevancy Score
97.2% Relevancy Score
97.1% Relevancy Score
94.1% Relevancy Score
93.5% Relevancy Score