Mouse monoclonal [J2D10] antibody to TNF alpha (Biotin).
Applications
IHC, IHC-P, ELISA
Positive Control
HeLa, HL-60, and A431 cellsor macrophages in lymph node and tonsil tissue.
Dilutions
Flow Cytometry: 0.5-1 µg/million cells in 100 µL, IF: 1-2 µg/mL. Directly conjugated primary antibodies may require higher working concentrations than indirect detection systems.
Reactivity
Human
Cross Reactivity
No cross-reactivity with Mouse. Cross-reactivity with other species has not been determined.
Immunogen
Recombinant human TNF alpha protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
J2D10
Isotype
IgG1
Light Chains
kappa
Conjugate
Biotin
Concentration
0.1 mg/mL
Predicted MW
17 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.05% Sodium Azide.
Storage
Shipped and stored at +4°C. This product is also photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. This CF® conjugated antibody is guaranteed for 2 years from data of receipt when stored correctly.
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