Mouse monoclonal [MAb11] antibody to TNF alpha (FITC).
Specificity
This antibody recognizes human 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests; Intracellular staining.
Reactivity
Porcine, Human, Non-Human Primates
Immunogen
Recombinant human TNF-alpha.
Host
Mouse
Clonality
Monoclonal
Clone ID
MAb11
Isotype
IgG1
Light Chains
kappa
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
17-26 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometry intracellular staining of human PHA stimulated peripheral blood mononuclear cells staining TNF alpha with Anti-TNF alpha Antibody [MAb11] (FITC) (A86711), (4 µl reagent per milion cells in 100 µl of cell suspension).
Flow cytometry multicolor intracellular staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using Anti-TNF alpha Antibody [MAb11] (FITC) (A86711), (4 µl reagent per milion cells in 100 µl of cell suspension). For surface staining was used Anti-CD3 Antibody [UCHT1] (APC) (A86518), (10 µl reagent per milion cells in 100 µl of cell suspension).
Separation of human TNF alpha positive CD3 positive cells (red-filled) from TNF alpha negative CD3 negative cells (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated peripheral blood mononuclear cells stained with Anti-TNF alpha Antibody [MAb11] (FITC) (A86711), (4 µl reagent per milion cells in 100 µl of cell suspension).
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Proteins predicted to interact with TNF alpha
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![Flow Cytometry - Anti-TNF alpha Antibody [MAb11] (FITC) (A86711)](https://cdn.antibodies.com/image/catalog/86/A86711_1.jpg?profile=product_top)
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