Anti-TNF alpha Antibody [MP6-XT3] (A281255)

Anti-TNF alpha Antibody [MP6-XT3]

Rat monoclonal [MP6-XT3] antibody to TNF alpha.

This antibody recognises mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha.

WB, Flow Cytometry, ELISA

ELISA: 5 µg/ml, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Mouse

Recombinant mouse TNF-alpha expressed in E. coli.

Rat

Monoclonal

MP6-XT3

IgG1

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse TNF alpha antibody, clone MP6-XT3 recognizes mouse tumor necrosis factor alpha (TNF alpha), also known as TNFSF2. This 17 kDa cytokine is produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26 kDa integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immunoregulatory role.Rat anti Mouse TNF alpha antibody, clone MP6-XT3 is reported to neutralize the effects of mouse TNF alpha (Bauman et al. 2003).

Cachectin, TNF, TNF-a, TNF-alpha, TNFA, TNFSF2, Tumor necrosis factor, Tumor necrosis factor ligand superfamily member 2