Anti-TOP2A (phospho Thr1343) Antibody (A95503)

This product is discontinued

Anti-TOP2A (phospho Thr1343) Antibody (A95503) has been discontinued and is no longer available.

View all TOP2A Antibodies.

Specifications

Name

Anti-TOP2A (phospho Thr1343) Antibody

Description

Rabbit polyclonal antibody to TOP2A (phospho Thr1343).

Specificity

This antibody detects endogenous levels of TOP2A only when phosphorylated at Thr1343.

Applications

WB, ELISA

Dilutions

WB: 1:500~1:1000, IHC: 1:50~1:100, ELISA: 1:20000

Reactivity

Human

Immunogen

Synthetic peptide derived from human TOP2A around the phosphorylation site of Thr1343 (amino acids 1311-1360).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

174kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

Western Blot - Anti-TOP2A (phospho Thr1343) Antibody (A95503)

Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40nM 30' using Anti-TOP2A (phospho Thr1343) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Western Blot - Anti-TOP2A (phospho Thr1343) Antibody (A95503)

Western blot analysis of 293T COLO using Anti-TOP2A (phospho Thr1343) Antibody.

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Proteins predicted to interact with TOP2A

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.

NUSAP1 Antibodies

99.9% Relevancy Score

CDK1 Antibodies

99.7% Relevancy Score

ASPM Antibodies

99.6% Relevancy Score

TOP1 Antibodies

99.4% Relevancy Score

Ki67 Antibodies

99.3% Relevancy Score

Cyclin B1 Antibodies

98.9% Relevancy Score

Bub1 Antibodies

98.2% Relevancy Score

Cyclin A2 Antibodies

98.2% Relevancy Score

BubR1 Antibodies

98% Relevancy Score