This antibody recognizes TRAIL, a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate, and also in many other tissues.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Recombinant fragment corresponding to amino acids 95-281 of human TRAIL protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
2E5
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Concentration
100 µg/ml
Predicted MW
21 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Apo-2 ligand, Apo-2L, APO2L, CD253, Protein TRAIL, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10
Anti-TRAIL Antibody [2E5] (PE) (A86216) specificity verification by flow cytometry. Colorectal cancer cell line HCT-116 with the eliminated expression of pro-apoptotic proteins BAK and BAX (prepared using CRISPR Cas9 gene editing approach) was used as host for CD253 (TRAIL) transfection. BAK/BAX double knockout cells are resistant to TRAIL-induced apoptosis. Anti-Hu CD253 PE (concentration in sample 5 µg/ml, red-filled histogram) binds specifically to surface CD253/TRAIL in transfected cells (upper panel), but not to the parent HCT-166 BAK/BAK double KO (lower panel). Level of non-specific binding was assessed using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199) under same conditions (concentration in sample 5 µg/ml, black-dashed histogram).
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Proteins predicted to interact with TRAIL
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
![WB - Anti-TRAIL Antibody [2E5] (PE) (A86216)](https://cdn.antibodies.com/image/catalog/86/A86216_1.jpg?profile=product_top)
![WB - Anti-TRAIL Antibody [2E5] (PE) (A86216)](https://cdn.antibodies.com/image/catalog/86/A86216_1.jpg?profile=product_top_thumb)
![WB - Anti-TRAIL Antibody [2E5] (PE) (A86216)](https://cdn.antibodies.com/image/catalog/86/A86216_1.jpg?profile=product_image)