This antibody recognizes TRAIL, a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate, and also in many other tissues.
Applications
Flow Cytometry, Functional Studies
Dilutions
FUNC: This antibody has high neutralizing activity for human TRAIL in biological assays, Flow Cytometry: 1-10 µg/ml.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse.
Immunogen
Recombinant fragment corresponding to amino acids 95-281 of human TRAIL protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
2E5
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
21 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Apo-2 ligand, Apo-2L, APO2L, CD253, Protein TRAIL, TNF-related apoptosis-inducing ligand, TNFSF10, Tumor necrosis factor ligand superfamily member 10
Functional application example: Apoptosis induced in JURKAT human T cell leukemia cell line by soluble recombinant human TRAIL is completely blocked by Anti-TRAIL Antibody [2E5] - Low endotoxin, Azide free (A86220). The neutralizing activity of Anti-TRAIL Antibody [2E5] - Low endotoxin, Azide free (A86220) has been confirmed with various sources of soluble recombinant human TRAIL. A - medium. B - recombinant TRAILC - recombinant TRAIL + Anti-TRAIL Antibody [2E5] - Low endotoxin, Azide free (A86220); 0.06 µg/ml. D - recombinant TRAIL + Anti-TRAIL Antibody [2E5] - Low endotoxin, Azide free (A86220); 0.24 µg/ml. E - recombinant TRAIL + Isotype mouse IgG1 control
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