Mouse monoclonal [24-31] antibody to TRAP/CD40L (APC).
Specificity
This antibody detects an extracellular epitope of CD154, a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human CD154 fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
24-31
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
39 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometry surface staining pattern of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells labeling TRAP/CD40L with Anti-TRAP/CD40L Antibody [24-31] (APC) (A320647), (10 µl reagent per million cells in 100 µl of cell suspension).
Flow cytometry multicolor surface staining of human lymphocytes using Anti-TRAP/CD40L Antibody [24-31] (APC) (A320647), (10 µl reagent per million cells in 100 µl of cell suspension) and Anti-CD3 Antibody [UCHT1] (PE) (A86520), (20 µl reagent per million cells in 100 µl of cell suspension).
Separation of human lymphocytes (red-filled) from blood debris (black-dashed) in flow cytometry surface staining of human stimulated (PHA + ionomycin) peripheral blood mononuclear cells, labeling TRAP/CD40L with Anti-TRAP/CD40L Antibody [24-31] (APC) (A320647), (10 µl reagent per million cells in 100 µl of cell suspension).
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