Mouse monoclonal [24-31] antibody to TRAP/CD40L (FITC).
Specificity
This antibody detects an extracellular epitope of CD154, a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Human CD154 fusion protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
24-31
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
39 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Separation of PMA/Ionomycine activated PBMCs stained with Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116), (4 µl reagent per million cells in 100 µl of cell suspension, red-filled) from PMA/Ionomycine activated PBMCs stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (FITC) (A86205), (concentration in sample 3 µg/ml, same as CD154 FITC concentration, black-dashed) in flow cytometry analysis (surface staining) of PMA/Ionomycine activated PBMCs.
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_1.jpg?profile=product_top)
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_2.jpg?profile=product_top)
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_1.jpg?profile=product_image)
![Flow Cytometry - Anti-TRAP/CD40L Antibody [24-31] (FITC) (A122116)](https://cdn.antibodies.com/image/catalog/122/A122116_2.jpg?profile=product_image)