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Anti-Ubiquitin (phospho Ser65) Antibody (A309718)

Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718) - Antibodies.com
$485
Product Datasheet

Rabbit polyclonal antibody to Ubiquitin (phospho Ser65) for WB.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 7-10 business days.

  • Specifications

Name
Anti-Ubiquitin (phospho Ser65) Antibody
Description
Rabbit polyclonal antibody to Ubiquitin (phospho Ser65).
Applications
WB
Dilutions
WB: 1:100-1:500
Reactivity
Mouse
Immunogen
A synthetic phosphorylated peptide around S65 of human Ubiquitin (NP_002945.1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Concentration
Lot Specific
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
Polyubiquitin-B, Polyubiquitin-C, UBB, UBC
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718) - Antibodies.comEnlarge Image

Western Blot - Anti-Ubiquitin (phospho Ser65) Antibody (A309718)

Western blot analysis of PC-3, using Anti-Ubiquitin (phospho Ser65) Antibody (A309718) at 1:400 dilution. PC-3 cells were treated by Carbonyl cyanide 3-chlorophenylhydrazone(10 uM) (10 µM) at 37°C for 6 hours after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
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Proteins predicted to interact with Ubiquitin

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score
99.9% Relevancy Score