This antibody recognizes CD106 antigen (VCAM-1), a 100-110 kDa type I membrane protein of the immunoglobulin superfamily, a crucial mediator of leukocyte adhesion, and a costimulation molecule.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human DS6 T cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
STA
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
100-110 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
CD106, INCAM-100, V-CAM 1, Vascular cell adhesion protein 1, VCAM-1
Separation of HDLM-2 cells stained with Anti-VCAM1 Antibody [STA] (PE) (A86139), (20 µl reagent per million cells in 100 µl of cell suspension, red-filled) from HDLM-2 cells stained using Mouse IgG1 kappa Isotype Control [MOPC-21] (PE) (A86199), (concentration in sample 3 µg/ml, same as CD106 PE concentration, black-dashed) in flow cytometry analysis (surface staining) of HDLM-2 cell suspension.
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