VCP expression in HeLa cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-VCP Antibody (A82483) at 0.3µg/ml and detected by chemiluminescence.
VCP expression in Human Prostate analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-VCP Antibody (A82483) at 5µg/ml and revealed with alkaline phosphatase (AP).
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