This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Applications
Flow Cytometry, WB, ICC
Dilutions
IHC: 10 µg/ml, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought), Flow Cytometry: Intraacrosomal staining: 3-12 µg/ml.
Reactivity
Mouse, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
Hs-14
Isotype
IgM
Conjugate
Unconjugated
Purification
Purified by differential precipitation and solid-phase chromatography.
Concentration
1 mg/ml
Predicted MW
220 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store at 2-8°C. Do not freeze!
Synonyms
15S Mg(2+)-ATPase p97 subunit, HEL-220, HEL-S-70, TER ATPase, Transitional endoplasmic reticulum ATPase, Valosin-containing protein
Immunocytochemistry staining of VCP in acetone-permeabilized human sperms (normal spermiogram) using Anti-VCP Antibody [Hs-14] (A86576) demonstrates its location to the acrosome.
Separation of human sperm cells stained with Anti-VCP Antibody [Hs-14] (A86576), (concentration in sample 9 µg/ml, GAM APC, red-filled) from human sperm cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).
![ICC - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_1.jpg?profile=product_top)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_2.jpg?profile=product_top)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_3.jpg?profile=product_top)
![ICC - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_1.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_2.jpg?profile=product_top_thumb)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_3.jpg?profile=product_top_thumb)
![ICC - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_1.jpg?profile=product_image)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_2.jpg?profile=product_image)
![Flow Cytometry - Anti-VCP Antibody [Hs-14] (A86576)](https://cdn.antibodies.com/image/catalog/86/A86576_3.jpg?profile=product_image)
