This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 50 tests. Intraacrosomal staining.
Reactivity
Mouse, Human
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Host
Mouse
Clonality
Monoclonal
Clone ID
Hs-14
Isotype
IgM
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
220 kDa
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 8, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
15S Mg(2+)-ATPase p97 subunit, HEL-220, HEL-S-70, TER ATPase, Transitional endoplasmic reticulum ATPase, Valosin-containing protein
Separation of human sperm cells stained with Anti-VCP Antibody [Hs-14] (FITC) (A121918), (concentration in sample 3 µg/ml, red) from unstained human sperm cells (black) in flow cytometry analysis (intracellular staining).
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Proteins predicted to interact with VCP
Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.