Anti-WIPI2 Antibody (A304859)

Immunohistochemistry analysis of human heart, fixed in formalin and paraffin-embedded. The Primary Antibody used was Anti-WIPI2 Antibody (A304859) at 1:50 for 30 minutes at room temperature. Counterstain: Hematoxylin. Magnification: 20X. HRP-DAB Detection.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-WIPI2 Antibody (A304859), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.

Western blot analysis of human HeLa and HEK293Trap cell lysates showing detection of ~49.4 kDa WIPI2 protein using Anti-WIPI2 Antibody (A304859) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:1,000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~49.4 kDa.