This antibody reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells. ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Applications
WB, ICC, Flow Cytometry (Intracellular)
Dilutions
Flow Cytometry: Intracellular staining: 2-5 µg/ml; Positive Control: HPB-ALL human peripheral blood T cell leukemia cell line, WB: 0.5-2 µg/ml.
Reactivity
Human
Immunogen
Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 protein with histidine tag.
Host
Mouse
Clonality
Monoclonal
Clone ID
ZAP-03
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
70 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western Blot - Anti-ZAP70 Antibody [ZAP-03] (A85696)
Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line. Lane 1, 2, 3, 4: Immunostaining with dilution range of Anti-ZAP70 Antibody (A85696); 4 µg/ml (1), 2 µg/ml (2), 1 µg/ml (3), 0.5 µg/ml (4). Lane 5: Immunostaining with Anti-ZAP-70 Anitbody (comparative antibody). Lane 6: Immunostaining with Isotype mouse IgG1 control (PPV-06).
Western Blot - Anti-ZAP70 Antibody [ZAP-03] (A85696)
Western Blotting analysis (reducing conditions) of HPB-ALL peripheral blood T cell leukemia cell line (1, 3) and RAMOS human Burkitt lymphoma cell line (2, 4). Lane 1, 2: Immunostaining with Anti-ZAP70 Antibody (A85696 | 0.5 µg/ml). Lane 3, 4: Immunostaining with Isotype mouse IgG1 control (PPV-06).
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