Anti-ZAP70 (phospho Tyr493) Antibody (A16675)

Western blot analysis of extracts of Jurkat cells, using Anti-ZAP70 (phospho Tyr493) Antibody (A16675) at 1:1,000 dilution. Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. Jurkat cells were treated by Pervanadate (1 mM) at 37°C for 30 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Anti-ZAP70 (phospho Tyr493) Antibody (A16675). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of Jurkat cells using Anti-ZAP70 (phospho Tyr493) Antibody (A16675) at a dilution of 1:100 (40x lens). Jurkat cells were treated by Hydrogen Peroxide (2 mM) at 37°C for 2 minutes after serum-starvation overnight. DAPI was used to stain the cell nuclei (blue).