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Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5 ng/ml corresponding to a specific activity of 200,000-10,000,000 IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF- must be present for observation of The Biological activity.
Purity
>98% as determined by SDS-PAGE.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from a solution containing 30% Acetonitrile and 0.1% TFA.
Storage
Shipped at ambient temperature. Lyophilized: Short term (3 weeks) store at 25°C. Long term store desiccated below -18°C. Reconstituted: Short term (2-7 days) store at 4°C. Long term store below -18°C. Avoid freeze/thaw cycles. Do not reconstite with neutral buffers. Do not use glass implements or extensive manipulations.
