One unit is defined as the amount of enzyme required to catalyze the release of 60pmol of uracil/minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50µl reaction containing 0.2µg DNA (104-105 cpm/µg) in 30 min. at 37°C.
Purity
>97% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
5U/µl
Formulation
Supplied in 10 mM Tris HCl Buffer, pH 7.4, with 50 mM Potassium Chloride, 100 µM EDTA, 1 mM DTT, 0.1 mg/ml BSA, and 50% Glycerol.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Synonyms
DGU, UDG, UNG1, UNG15, Uracil-DNA glycosylase
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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