One unit is defined as the amount of enzyme that releases 1 nmol of uracils from the DNA strand (containing dU) within 1 hour at 37°C in the reaction system containing 70mM TrisHCl, pH -7.5, 10mM NaCl, 1mM EDTA and 0.1mg/ml BSA reaction liquid.
Purity
>97% as determined by SDS-PAGE.
Product Form
Liquid
Concentration
1U/µl
Formulation
Supplied in 20 mM Tris HCl Buffer, pH 8, with 100 mM Potassium Chloride, 100 µM EDTA, 1 mM DTT, 0.5% NP-40, 0.5% Tween 20, and 50% Glycerol.
Storage
Shipped at 4°C. Short term (2-4 weeks) store at 4°C. Long term store at -20°C, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles.
Synonyms
DGU, UDG, UNG1, UNG15, Uracil-DNA glycosylase
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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