This protein is constructed from the following CCR4 sequences: (Met1-Glu39) (Ala99-Lys111) (Ser176-Val200) (Val268-Tyr284). The human Fc tag consists of amino acids Glu99-Ala330.
The protein has a predicted molecular mass of 37.1 kDa after removal of the signal peptide. The apparent molecular mass of CCR4-hFc is approximately 35-55 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Reconstitute with distilled sterile water.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with Recombinant Human CCR4 Protein (Fc Tag) (A318307) at 2 µg/ml (100 µl/well) which can bind Anti-CCR4 Antibody [DMC297] - BSA and Azide free (A318770) in a linear range of 0.64-16 ng/ml.
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