Synthetic nanodiscs offer a stable and biologically relevant environment that closely mimics cell membranes and enables full-length transmembrane human CCR4 protein to be purified and analysed in vitro.
Applications
ELISA
Expression System
HEK293 cells
Nature
Synthetic
Protein Species
Human
Protein Length
Full length protein.
Molecular Weight
Full length human CCR4 protein has a MW of 41.4 kDa.
Conjugate
Unconjugated
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Reconstitute with distilled sterile water.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
ELISA plates were pre-coated with 0.5 µg/per well Synthetic Nanodisc Human CCR4 Protein (A318482). Serial diluted Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) solutions were added, washed, and incubated with secondary antibody before ELISA reading. From above data, the EC50 for Anti-CCR4 Humanized Antibody [Mogamulizumab Biosimilar] - Azide free (A318876) binding with CCR4-VLP is 856.2 µg/ml.
Diagram showing how synthetic nanodiscs containing full-length multi-pass transmembrane proteins in a phospholipid bilayer are generated from native cell membranes.
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