The protein has a predicted molecular mass of 69.4 kDa after removal of the signal peptide. The apparent molecular mass of EGFR-His is approximately 100-130 kDa due to glycosylation.
Conjugate
Unconjugated
Purity
> 95% by SDS-PAGE and Coomassie blue staining.
Product Form
Lyophilized
Concentration
Reconstitution dependent.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Reconstitution
Reconstitute with distilled sterile water.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Recombinant Human EGFR Protein (6xHis Tag) (A318318)
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human EGFR Protein (6×His Tag) (A318318) which can bind Anti-EGFR Chimeric Antibody [Cetuximab Biosimilar] - Azide free (A318922) in a linear range of 0.64-80 µg/ml.
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